Molecular Biology

Diagnostic molecular biology is the analysis of DNA or RNA with Conventional Polymerase Chain Reaction (PCR), Real-time PCR and High-throughput DNA sequencing techniques. These techniques are used in clinical/risk diagnosis and diagnostic monitoring. Our team of experienced specialists at BRIA LAB will use up-to-date technologies to deliver fast and accurate results so if necessary, you can get your treatment as soon as possible.

BRIA LAB’s Diagnostic molecular biology services include:

  1. HIV VIRAL LOAD (RT-PCR)

    BRIA LAB uses plasma kept in EDTA tubes to do HIV-1 RNA levels screening. This screening is a prognosis of progression and results of antiviral drugs’ reactions. This testing does not intend to conduct further research on viral infections or do an HIV-1 confirmatory test.

    HIV-1 RNA levels screening is conducted by extracting viral RNA and increasing RNA levels with the real time PCR technique. This HIV-1 RNA levels screening is operated from 20-10,000,000 copies/mL, for 1 copy of HIV-1 RNA equals to 1.7 IU according to WHO standard (NIBC code 97/656.)

  2. HBV VIRAL LOAD (RT-PCR)

    HBV preserved in serum or plasma is kept in an EDTA tube. The serum or plasma’s DNA levels are screened to monitor patients with chronic Hepatitis B. This testing is not intended to for use in blood screening or the screening in blood products for viral infection or to confirm the presence of Hepatitis B.

    HBV DNA levels screening is conducted by extracting viral DNA and increasing DNA levels with the real time PCR technique. This HBV DNA levels screening can be operated with Genotype A-H, and pre-core from 20-170,000,000 IU/mL, for 1 IU of HBV DNA equals to 5.82 Cp.

  3. HCV VIRAL LOAD (RT-PCR)

    The screening of RNA levels in HCV preserved in serum or plasma kept in EDTA tube is done for monitoring patients who take antiviral drugs. This testing is not intended to be used in blood screening or any other screenings of blood products to conduct further research on viral infections or to confirm the presence of Hepatitis C.

    HCV RNA levels screening is conducted by extracting viral RNA and increasing RNA levels using real time PCR technique. This HBV RNA levels screening can be operated from 15-100,000,000 IU/mL.

  4. TB PCR

    Mycobacteria Tuberculosis (MTB) complex (M.tuberculosis, M.bovis, M.africanum, and M.microti) can be screened with the Real-Time PCR technique. BRIA LAB can get a faster and more accurate diagnosis using a 16S rRNA test of the MTB complex rather than testing with bacterial culture. With this technique, we can deliver results to get patients treatment sooner.

  5. InfluA (H1N1 2009)

    BRIA LAB can screen for InfluA (H1N1 2009) by using the One Step Real-Time PCR technique or the Conventional PCR technique. The test specifically screens the matrix protein (M2) and Hemagglutinnin (H1) of H1N1 2009 influenza virus. The testing is done on samples extracted from a throat swab, Nasopharyngeal swab, Nasal swab, and Nasopharyngeal wash.

  6. DENGUE Serotypes

    The viruses that cause Dengue and Hemorrhagic Dengue Fever can be carried and transmitted by a common house mosquito. The virus can be categorized by 4 serotypes and can be screened using the PCR Technique. BRIA LAB can confirm that Dengue fever is present and identify the serotype, which is significant to surveillance of Dengue Hemorrhagic Fever.

  7. PCR for Thalassemia (alpha 1&2)

    Thalassemia is a form of genetic disorders caused by globin gene mutations. Thalassemia can be categorized into 2 major types, according to the globin types, including α –thalassemia and β–thalassemia. The screening of α –thalassemia using Multiplex Polymerase Chain Reaction can be conducted by testing the absences of α1 and α2 globin genes of chromosome 16. The absences can be categorized into several different types, including SEA, THAI, MED, FIL 20.5 kb, 3.7 kb, and 4.2 kb deletion type.

  8. PCR for HSVI&II

    Herpes is a skin disease caused by herpes simplex virus, or HSV, that can be categorized into 2 types: type 1 and type 2. The screening for herpes simplex virus DNA can be conducted using conventional PCR technique and Real time PCR technique from specimens that are from a genital swab sample, herpes secretion sample, CSF or serum.

  9. PCR for JAK2 V617F Mutation

    The screening of JAK2 V617F Mutation using allele-specific PCR technique is a mutation screening on nucleotide 617 with amino acid replacement from Valine (V) to Phenylalanine (F). The screening follows the standard of myeloproliferative disorders (MPD) diagnosis or disorders caused by abnormalities of hematopoietic stem cells where blood cells are increasingly and over abundantly produced. MPD consists of 3 main diseases including Polycythemia Vera (PV), Essential Thrombocythemia (ET) and Idiopathic Myelofibrosis (IMF).

  10. NIFTY หรือ Non-Invasive Fetal Trisomy test

    The Non-Invasive Fetal Trisomy Test is an efficient way to do prenatal screening for Fetal Down Syndrome. It uses high-throughput DNA sequencing technology. The screening can detect the Trisomy 21 type of Fetal Down Syndrome with 99% accuracy. Most other commonly used testing methods are only 60-85% reliable. Additionally, this screening also helps reduce risks of miscarriage after extrinsic amniocentesis.

Essential documents for laboratory test

Request and Consent Forms

Research Papers